› 3e8x

X-ray diffraction
2.1Å resolution

Putative NAD-dependent epimerase/dehydratase from Bacillus halodurans.

Released:
DOI: 10.2210/pdb3e8x/pdb
PDB entry 3e8x coloured by chain, front view.
PDB entry 3e8x coloured by chain, side view.
PDB entry 3e8x coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry authors: Osipiuk J, Skarina T, Onopriyenko O, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.21 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KCP9 (Residues: 1-213; Coverage: 100%)
Gene name: BH1520
Sequence domains: NAD(P)H-binding
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAP 1 x NAP
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 113.687Å b: 113.687Å c: 63.645Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.193
Expression system: Escherichia coli

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