› 3e99

X-ray diffraction
1.9Å resolution

Crystal structure of the beta subunit of the benzoate 1,2-dioxygenase (benb, bmaa0186) from burkholderia mallei atcc 23344 at 1.90 A resolution

Released:
DOI: 10.2210/pdb3e99/pdb
PDB entry 3e99 coloured by chain, front view.
PDB entry 3e99 coloured by chain, side view.
PDB entry 3e99 coloured by chain, top view.
Source organism: Burkholderia mallei ATCC 23344
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Benzoate + NADH + O(2) = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoate 1,2-dioxygenase beta subunit Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 19.35 KDa
Source organism: Burkholderia mallei ATCC 23344
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2WBM4 (Residues: 1-163; Coverage: 100%)
Gene names: BMAA0186, benB
Sequence domains: Ring hydroxylating beta subunit
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6322
Unit cell:
a: 58.953Å b: 58.953Å c: 167.073Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.223
Expression system: Escherichia coli

Quick links