› 3e9a

X-ray diffraction
1.8Å resolution

Crystal structure of 2-dehydro-3-deoxyphosphooctonate aldolase from Vibrio cholerae O1 biovar eltor str. N16961

Released:
DOI: 10.2210/pdb3e9a/pdb
PDB entry 3e9a coloured by chain, front view.
PDB entry 3e9a coloured by chain, side view.
PDB entry 3e9a coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor
Entry authors: Nocek B, Mulligan R, Kwon K, Joachimiak A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphooctonate aldolase Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 31.08 KDa
Source organism: Vibrio cholerae O1 biovar El Tor
Expression system: Escherichia coli
UniProt:
  • Canonical: A5F692 (Residues: 1-283; Coverage: 100%)
Gene names: VC0395_A1752, VC395_2289, kdsA
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I23
Unit cell:
a: 117.187Å b: 117.187Å c: 117.187Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Escherichia coli

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