› 3e9d

X-ray diffraction
2Å resolution

Structure of full-length TIGAR from Danio rerio

Released:
DOI: 10.2210/pdb3e9d/pdb
PDB entry 3e9d coloured by chain, front view.
PDB entry 3e9d coloured by chain, side view.
PDB entry 3e9d coloured by chain, top view.
Source organism: Danio rerio
Entry authors: Li H, Jogl G

Function and Biology Details

Reaction catalysed: 
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-2,6-bisphosphatase TIGAR B Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 29.61 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7ZVE3 (Residues: 1-257; Coverage: 100%)
Gene names: si:ch211-240j22.3, tigarb, zgc:56074
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: C2
Unit cell:
a: 127.661Å b: 115.541Å c: 61.311Å
α: 90° β: 104.66° γ: 90°
R-values:
R R work R free
0.173 0.171 0.213
Expression system: Escherichia coli

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