› 3e9o

X-ray diffraction
2Å resolution

Crystal Structure of Yeast Prp8, Residues 1836-2092

Released:
DOI: 10.2210/pdb3e9o/pdb
Model geometry
Fit model/data
PDB entry 3e9o coloured by chain, front view.
PDB entry 3e9o coloured by chain, side view.
PDB entry 3e9o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and function of an RNase H domain at the heart of the spliceosome.
Pena V, Rozov A, Fabrizio P, Lührmann R, Wahl MC
EMBO J 27 2929-40 (2008)
PMID: 18843295

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33334 (Residues: 1836-2087; Coverage: 10%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PRP8 domain IV core
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P31
Unit cell:
a: 46.857Å b: 46.857Å c: 102.995Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.32 0.177 0.245
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
18 more

3 mentions without citation

Structure and function of an RNase H domain at the heart of the spliceosome.
Pena et al. (2008)
2 more