› 3eaa

X-ray diffraction
2.79Å resolution

Structure of a type six secretion system protein

Released:
DOI: 10.2210/pdb3eaa/pdb
PDB entry 3eaa coloured by chain, front view.
PDB entry 3eaa coloured by chain, side view.
PDB entry 3eaa coloured by chain, top view.
Source organism: Edwardsiella tarda
Primary publication:
Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiella tarda.
Jobichen C, Chakraborty S, Li M, Zheng J, Joseph L, Mok YK, Leung KY, Sivaraman J
PLoS One 5 e12910 (2010)
PMID: 20886112

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EvpC Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.12 KDa
Source organism: Edwardsiella tarda
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6EE19 (Residues: 1-163; Coverage: 100%)
Gene name: evpC
Sequence domains: Type VI secretion system effector, Hcp
Structure domains: Hcp1-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6
Unit cell:
a: 85.459Å b: 85.459Å c: 93.066Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.238 0.284
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Structure and regulation of the type VI secretion system.
Silverman et al. (2012)
10 more

15 mentions without citation

A common evolutionary origin for tailed-bacteriophage functional modules and bacterial machineries.
Veesler et al. (2011)
14 more