› 3eam

X-ray diffraction
2.9Å resolution

An open-pore structure of a bacterial pentameric ligand-gated ion channel

Released:
DOI: 10.2210/pdb3eam/pdb
PDB entry 3eam coloured by chain, front view.
PDB entry 3eam coloured by chain, side view.
PDB entry 3eam coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
X-ray structure of a pentameric ligand-gated ion channel in an apparently open conformation.
Bocquet N, Nury H, Baaden M, Le Poupon C, Changeux JP, Delarue M, Corringer PJ
Nature 457 111-4 (2009)
PMID: 18987633

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 317 amino acids
Theoretical weight: 36.29 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 43-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LMT 6 x LMT
Ligand PC1 15 x PC1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 183.32Å b: 133.64Å c: 160.18Å
α: 90° β: 102.94° γ: 90°
R-values:
R R work R free
0.2 0.199 0.231
Expression system: Escherichia coli

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Citations

107 review citations

Nicotinic receptors: allosteric transitions and therapeutic targets in the nervous system.
Taly et al. (2009)
106 more

91 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
90 more