› 3eb7

X-ray diffraction
2.3Å resolution

Crystal Structure of Insecticidal Delta-Endotoxin Cry8Ea1 from Bacillus Thuringiensis at 2.2 Angstroms Resolution

Released:
DOI: 10.2210/pdb3eb7/pdb
PDB entry 3eb7 coloured by chain, front view.
PDB entry 3eb7 coloured by chain, side view.
PDB entry 3eb7 coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela.
Guo S, Ye S, Liu Y, Wei L, Xue J, Wu H, Song F, Zhang J, Wu X, Huang D, Rao Z
J Struct Biol 168 259-66 (2009)
PMID: 19591941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crystaline entomocidal protoxin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 589 amino acids
Theoretical weight: 66.3 KDa
Source organism: Bacillus thuringiensis
Expression system: Bacillus thuringiensis
UniProt:
  • Canonical: B3LEP7 (Residues: 64-652; Coverage: 51%)
Gene names: D7J84_27750, cry8E2, cry8Ea1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 407.282Å b: 47.956Å c: 103.121Å
α: 90° β: 91.12° γ: 90°
R-values:
R R work R free
0.171 0.162 0.23
Expression system: Bacillus thuringiensis

Quick links

Citations

11 review citations

Bacillus thuringiensis: A story of a successful bioinsecticide.
Bravo et al. (2011)
10 more

15 mentions without citation

Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins.
Xu et al. (2014)
14 more