› 3ebv

X-ray diffraction
1.5Å resolution

Crystal structure of putative Chitinase A from Streptomyces coelicolor.

Released:
DOI: 10.2210/pdb3ebv/pdb
PDB entry 3ebv coloured by chain, front view.
PDB entry 3ebv coloured by chain, side view.
PDB entry 3ebv coloured by chain, top view.
Source organism: Streptomyces coelicolor
Entry authors: Vigdorovich V, Ramagopal UA, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 32.09 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z9M9 (Residues: 280-570; Coverage: 54%)
Gene name: chiA
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 96.636Å b: 96.636Å c: 124.735Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.169
Expression system: Escherichia coli BL21(DE3)

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