› 3ecp

X-ray diffraction
2.5Å resolution

Crystal Structure Of Tn5 Transposase Complexed With 5' Phosphorylated Transposon End DNA

Released:
DOI: 10.2210/pdb3ecp/pdb
PDB entry 3ecp coloured by chain, front view.
PDB entry 3ecp coloured by chain, side view.
PDB entry 3ecp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif.
Klenchin VA, Czyz A, Goryshin IY, Gradman R, Lovell S, Rayment I, Reznikoff WS
Nucleic Acids Res 36 5855-62 (2008)
PMID: 18790806

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116204 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase for transposon Tn5 Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 53.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46731 (Residues: 1-476; Coverage: 100%)
Gene names: tnp, tnpA
Sequence domains:
Structure domains:
DNA transferred strand Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA non-transferred strand Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.05 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 113.508Å b: 113.508Å c: 228.775Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.219 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

DNA Transposition at Work.
Hickman et al. (2016)
3 more

3 mentions without citation

DDE transposases: Structural similarity and diversity.
Nesmelova et al. (2010)
2 more