› 3edc

X-ray diffraction
2.1Å resolution

Crystal Structure of a 1.6-hexanediol Bound Tetrameric Form of Escherichia coli Lac-repressor Refined to 2.1 Resolution

Released:
DOI: 10.2210/pdb3edc/pdb
PDB entry 3edc coloured by chain, front view.
PDB entry 3edc coloured by chain, side view.
PDB entry 3edc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of a 1.6-hexanediol bound tetrameric form of Escherichia coli lac-repressor refined to 2.1 A resolution.
Stenberg KA, Vihinen M
Proteins 75 748-59 (2009)
PMID: 19004002

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 38.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-360; Coverage: 100%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand HEZ 4 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 164.15Å b: 72.434Å c: 130.566Å
α: 90° β: 113.96° γ: 90°
R-values:
R R work R free
0.159 0.156 0.201
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural insights into the role of architectural proteins in DNA looping deduced from computer simulations.
Olson et al. (2013)
5 other articles

6 mentions without citation

Creation of artificial protein-protein interactions using α-helices as interfaces.
Yagi et al. (2018)
5 more