› 3eee

X-ray diffraction
2.12Å resolution

Probing the function of heme distortion in the H-NOX family

Released:
DOI: 10.2210/pdb3eee/pdb
PDB entry 3eee coloured by chain, front view.
PDB entry 3eee coloured by chain, side view.
PDB entry 3eee coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Probing the function of heme distortion in the H-NOX family.
Olea C, Boon EM, Pellicena P, Kuriyan J, Marletta MA
ACS Chem Biol 3 703-10 (2008)
PMID: 19032091

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting transducer domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 188 amino acids
Theoretical weight: 22.02 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RBX6 (Residues: 1-188; Coverage: 31%)
Gene names: TTE0680, Tar4
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

4 bound ligands:
Ligand HEM 4 x HEM
Ligand OXY 4 x OXY
Ligand SO4 3 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 116.253Å b: 124.686Å c: 83.555Å
α: 90° β: 126.58° γ: 90°
R-values:
R R work R free
0.207 0.205 0.252
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
11 more

9 mentions without citation

Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor.
Klopfenstein et al. (2002)
8 more