› 3eef

X-ray diffraction
2.35Å resolution

Crystal structure of N-carbamoylsarcosine amidase from thermoplasma acidophilum

Released:
DOI: 10.2210/pdb3eef/pdb
PDB entry 3eef coloured by chain, front view.
PDB entry 3eef coloured by chain, side view.
PDB entry 3eef coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Primary publication:
Crystal structure and molecular modeling study of N-carbamoylsarcosine amidase Ta0454 from Thermoplasma acidophilum.
Luo HB, Zheng H, Zimmerman MD, Chruszcz M, Skarina T, Egorova O, Savchenko A, Edwards AM, Minor W
J Struct Biol 169 304-11 (2010)
PMID: 19932181

Function and Biology Details

Reaction catalysed: 
N-carbamoylsarcosine + H(2)O = sarcosine + CO(2) + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismatase-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.79 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HKY9 (Residues: 1-182; Coverage: 100%)
Gene name: Ta0454
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P62
Unit cell:
a: 83.97Å b: 83.97Å c: 98.725Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Characterizing metal-binding sites in proteins with X-ray crystallography.
Handing et al. (2018)
4 more

5 mentions without citation

In situ proteolysis to generate crystals for structure determination: an update.
Wernimont et al. (2009)
4 more