› 3eeg

X-ray diffraction
2.78Å resolution

Crystal structure of a 2-isopropylmalate synthase from Cytophaga hutchinsonii

Released:
DOI: 10.2210/pdb3eeg/pdb
PDB entry 3eeg coloured by chain, front view.
PDB entry 3eeg coloured by chain, side view.
PDB entry 3eeg coloured by chain, top view.
Source organism: Cytophaga hutchinsonii ATCC 33406
Entry authors: Sugadev R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-isopropylmalate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 325 amino acids
Theoretical weight: 36.14 KDa
Source organism: Cytophaga hutchinsonii ATCC 33406
Expression system: Escherichia coli BL21(DE3)
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6522
Unit cell:
a: 118.666Å b: 118.666Å c: 154.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.245 0.298
Expression system: Escherichia coli BL21(DE3)

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