› 3ees

X-ray diffraction
1.9Å resolution

Structure of the RNA pyrophosphohydrolase BdRppH

Released:
DOI: 10.2210/pdb3ees/pdb
PDB entry 3ees coloured by chain, front view.
PDB entry 3ees coloured by chain, side view.
PDB entry 3ees coloured by chain, top view.
Source organism: Bdellovibrio bacteriovorus
Primary publication:
Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
Messing SA, Gabelli SB, Liu Q, Celesnik H, Belasco JG, Piñeiro SA, Amzel LM
Structure 17 472-81 (2009)
PMID: 19278661

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.63 KDa
Source organism: Bdellovibrio bacteriovorus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6MPX4 (Residues: 1-153; Coverage: 100%)
Gene names: Bd0714, mutT
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P3221
Unit cell:
a: 70.491Å b: 70.491Å c: 100.481Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New aspects of RNA processing in prokaryotes.
Evguenieva-Hackenberg et al. (2011)
1 more

1 mention without citation

Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
Messing et al. (2009)