› 3eey

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF PUTATIVE RRNA-METHYLASE FROM Clostridium thermocellum

Released:
DOI: 10.2210/pdb3eey/pdb
PDB entry 3eey coloured by chain, front view.
PDB entry 3eey coloured by chain, side view.
PDB entry 3eey coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Entry authors: Patskovsky Y, Ramagopal UA, Toro R, Rutter M, Hu S, Bain K, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA methylase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 197 amino acids
Theoretical weight: 21.84 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DDA2 (Residues: 3-188; Coverage: 99%)
Gene name: Cthe_0696
Sequence domains: Putative rRNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
Ligand SAM 9 x SAM
Ligand SO4 5 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 70.156Å b: 126.913Å c: 125.611Å
α: 90° β: 99.54° γ: 90°
R-values:
R R work R free
0.245 0.244 0.278
Expression system: Escherichia coli

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