› 3ef2

X-ray diffraction
1.8Å resolution

Structure of the Marasmius oreades mushroom lectin (MOA) in complex with Galalpha(1,3)[Fucalpha(1,2)]Gal and Calcium.

Released:
DOI: 10.2210/pdb3ef2/pdb
PDB entry 3ef2 coloured by chain, front view.
PDB entry 3ef2 coloured by chain, side view.
PDB entry 3ef2 coloured by chain, top view.
Source organism: Marasmius oreades
Primary publication:
Structural characterization of a lectin from the mushroom Marasmius oreades in complex with the blood group B trisaccharide and calcium.
Grahn EM, Winter HC, Tateno H, Goldstein IJ, Krengel U
J Mol Biol 390 457-66 (2009)
PMID: 19426740

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Agglutinin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 32.38 KDa
Source organism: Marasmius oreades
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X123 (Residues: 1-293; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GLA
Carbohydrate polymer
2 bound ligands:
Ligand CA 16 x CA
Ligand ACT 4 x ACT
1 modified residue:
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3
Unit cell:
a: 121.279Å b: 121.279Å c: 100.124Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.189
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mushroom lectins: current status and future perspectives.
Singh et al. (2010)
3 more

9 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
8 more