3efo

X-ray diffraction
2.7Å resolution

Crystal Structure of the mammalian COPII-coat protein Sec23/24 bound to the transport signal sequence of syntaxin 5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-131777 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein Sec23A Chain: A
Molecule details ›
Chain: A
Length: 765 amino acids
Theoretical weight: 86.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15436 (Residues: 1-765; Coverage: 100%)
Gene name: SEC23A
Sequence domains:
Structure domains:
Protein transport protein Sec24D Chain: B
Molecule details ›
Chain: B
Length: 770 amino acids
Theoretical weight: 86.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O94855 (Residues: 266-1032; Coverage: 74%)
Gene names: KIAA0755, SEC24D
Sequence domains:
Structure domains:
Syntaxin-5 Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 794 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13190 (Residues: 242-248; Coverage: 2%)
Gene names: STX5, STX5A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 103.06Å b: 140.83Å c: 152.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.261
Expression systems:
  • Spodoptera frugiperda
  • Not provided