3egc

X-ray diffraction
2.35Å resolution

Crystal structure of a putative ribose operon repressor from Burkholderia thailandensis

Released:
Entry authors: Bonanno JB, Patskovsky Y, Gilmore M, Bain KT, Hu S, Romero R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-173992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 291 amino acids
Theoretical weight: 31.88 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2T0D1 (Residues: 61-343; Coverage: 83%)
Gene name: BTH_I0812
Sequence domains: Periplasmic binding proteins and sugar binding domain of LacI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 50.712Å b: 288.651Å c: 63.355Å
α: 90° β: 113.59° γ: 90°
R-values:
R R work R free
0.259 0.257 0.299
Expression system: Escherichia coli BL21(DE3)