› 3ejw

X-ray diffraction
1.8Å resolution

Crystal Structure of the Sinorhizobium meliloti AI-2 receptor, SmLsrB

Released:
DOI: 10.2210/pdb3ejw/pdb
PDB entry 3ejw coloured by chain, front view.
PDB entry 3ejw coloured by chain, side view.
PDB entry 3ejw coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Primary publication:
Sinorhizobium meliloti, a bacterium lacking the autoinducer-2 (AI-2) synthase, responds to AI-2 supplied by other bacteria.
Pereira CS, McAuley JR, Taga ME, Xavier KB, Miller ST
Mol Microbiol 70 1223-35 (2008)
PMID: 18990189

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer 2-binding protein LsrB Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 33.9 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q926H7 (Residues: 29-343; Coverage: 100%)
Gene name: SM_b21016
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PAV 2 x PAV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 57.848Å b: 71.488Å c: 68.035Å
α: 90° β: 98.69° γ: 90°
R-values:
R R work R free
0.17 0.168 0.214
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Oral multispecies biofilm development and the key role of cell-cell distance.
Kolenbrander et al. (2010)
9 more

4 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
3 more