› 3eld

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA cap 0-1 NS5-type MT domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 34.19 KDa
Source organism: Wesselsbron virus
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX01 (Residues: 1-275; Coverage: 100%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SFG 1 x SFG
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 47.67Å b: 66.132Å c: 88.495Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.224
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural insights into SARS-CoV-2 proteins.
Arya et al. (2021)
11 more

3 mentions without citation

Structure and functionality in flavivirus NS-proteins: perspectives for drug design.
Bollati et al. (2010)
2 more