› 3eo8

X-ray diffraction
1.74Å resolution

Crystal structure of BluB-like flavoprotein (YP_001089088.1) from CLOSTRIDIUM DIFFICILE 630 at 1.74 A resolution

Released:
DOI: 10.2210/pdb3eo8/pdb
PDB entry 3eo8 coloured by chain, front view.
PDB entry 3eo8 coloured by chain, side view.
PDB entry 3eo8 coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 219 amino acids
Theoretical weight: 25.13 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli
UniProt:
  • Canonical: Q182R2 (Residues: 1-218; Coverage: 100%)
Gene name: CD630_25720
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

4 bound ligands:
Ligand FMN 6 x FMN
Ligand ACT 12 x ACT
Ligand CL 6 x CL
Ligand GOL 7 x GOL
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 51.843Å b: 183.265Å c: 74.393Å
α: 90° β: 104.89° γ: 90°
R-values:
R R work R free
0.159 0.156 0.198
Expression system: Escherichia coli

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