› 3eoe

X-ray diffraction
2.31Å resolution

Crystal Structure of Pyruvate Kinase from toxoplasma gondii, 55.m00007

Released:
DOI: 10.2210/pdb3eoe/pdb
PDB entry 3eoe coloured by chain, front view.
PDB entry 3eoe coloured by chain, side view.
PDB entry 3eoe coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
The crystal structure of Toxoplasma gondii pyruvate kinase 1.
Bakszt R, Wernimont A, Allali-Hassani A, Mok MW, Hills T, Hui R, Pizarro JC
PLoS One 5 e12736 (2010)
PMID: 20856875

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 511 amino acids
Theoretical weight: 55.93 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q969A2 (Residues: 39-531; Coverage: 93%)
Gene names: TGRH88_072970, pk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 98.039Å b: 130.684Å c: 113.848Å
α: 90° β: 117.4° γ: 90°
R-values:
R R work R free
0.208 0.206 0.253
Expression system: Escherichia coli

Quick links

Citations

2 review citations

An overview of structure, function, and regulation of pyruvate kinases.
Schormann et al. (2019)
1 more

2 mentions without citation

The crystal structure of Toxoplasma gondii pyruvate kinase 1.
Bakszt et al. (2010)
1 more