› 3epd

Electron Microscopy
9Å resolution

CryoEM structure of poliovirus receptor bound to poliovirus type 3

Released:
DOI: 10.2210/pdb3epd/pdb
PDB entry 3epd coloured by chain, front view.
PDB entry 3epd coloured by chain, side view.
PDB entry 3epd coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses.
Zhang P, Mueller S, Morais MC, Bator CM, Bowman VD, Hafenstein S, Wimmer E, Rossmann MG
Proc Natl Acad Sci U S A 105 18284-9 (2008)
PMID: 19011098
Related structures: EMD-1562

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
PDBe Complex ID:
PDB-CPX-100961 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Poliovirus receptor Chain: R
Molecule details ›
Chain: R
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15151 (Residues: 30-242; Coverage: 54%)
Gene names: PVR, PVS
Sequence domains:
Poliovirus Type3 peptide Chain: 0
Molecule details ›
Chain: 0
Length: 4 amino acids
Theoretical weight: 446 Da
protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 279 amino acids
Theoretical weight: 31.36 KDa
Source organism: Poliovirus 3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B6VQW0 (Residues: 43-321; Coverage: 78%)
Sequence domains: picornavirus capsid protein
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 266 amino acids
Theoretical weight: 29.64 KDa
Source organism: Poliovirus 3
Expression system: Escherichia coli
UniProt:
  • Canonical: P03302 (Residues: 75-340; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 68 amino acids
Theoretical weight: 7.32 KDa
Source organism: Poliovirus 3
Expression system: Escherichia coli
UniProt:
  • Canonical: P03302 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 235 amino acids
Theoretical weight: 25.95 KDa
Source organism: Poliovirus 3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4MVN6 (Residues: 341-575; Coverage: 11%)
Sequence domains: picornavirus capsid protein

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9Å
Relevant EMDB volumes: EMD-1562
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Connections matter--how viruses use cell–cell adhesion components.
Mateo et al. (2015)
6 more

3 mentions without citation

Antivirals blocking entry of enteroviruses and therapeutic potential.
Anasir et al. (2021)
2 more