› 3eqe

X-ray diffraction
2.82Å resolution

Crystal structure of the YubC protein from Bacillus subtilis. Northeast Structural Genomics Consortium target SR112.

Released:
DOI: 10.2210/pdb3eqe/pdb
PDB entry 3eqe coloured by chain, front view.
PDB entry 3eqe coloured by chain, side view.
PDB entry 3eqe coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Vorobiev SM, Su M, Seetharaman J, Benach J, Forouhar F, Clayton G, Cooper B, Wang H, Foote EL, Ciccosanti C, Mao L, Xiao R, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 19.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32085 (Residues: 1-161; Coverage: 100%)
Gene names: BSU31140, cdoA, yubC
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P4322
Unit cell:
a: 65.83Å b: 65.83Å c: 197.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.296
Expression system: Escherichia coli

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