› 3es8

X-ray diffraction
2.2Å resolution

Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.

Released:
DOI: 10.2210/pdb3es8/pdb
PDB entry 3es8 coloured by chain, front view.
PDB entry 3es8 coloured by chain, side view.
PDB entry 3es8 coloured by chain, top view.
Source organism: Oceanobacillus iheyensis
Primary publication:
Computation-facilitated assignment of the function in the enolase superfamily: a regiochemically distinct galactarate dehydratase from Oceanobacillus iheyensis .
Rakus JF, Kalyanaraman C, Fedorov AA, Fedorov EV, Mills-Groninger FP, Toro R, Bonanno J, Bain K, Sauder JM, Burley SK, Almo SC, Jacobson MP, Gerlt JA
Biochemistry 48 11546-58 (2009)
PMID: 19883118

Function and Biology Details

Reaction catalysed: 
Galactarate = (2S,3R)-2,3-dihydroxy-5-oxohexanedioate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactarate dehydratase (D-threo-forming) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 391 amino acids
Theoretical weight: 44.4 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EMJ9 (Residues: 1-391; Coverage: 100%)
Gene name: OB2843
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LMR 8 x LMR
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P1
Unit cell:
a: 105.584Å b: 105.695Å c: 105.718Å
α: 109.31° β: 109.47° γ: 109.66°
R-values:
R R work R free
0.226 0.224 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Enzyme Function Initiative.
Gerlt et al. (2011)
2 more

3 mentions without citation

Structural mechanism of trimeric HIV-1 envelope glycoprotein activation.
Tran et al. (2012)
2 more