› 3esa

X-ray diffraction
2Å resolution

cut-1b; NCN-Pt-Pincer-Cutinase Hybrid

Released:
DOI: 10.2210/pdb3esa/pdb
PDB entry 3esa coloured by chain, front view.
PDB entry 3esa coloured by chain, side view.
PDB entry 3esa coloured by chain, top view.
Source organism: Fusarium vanettenii
Primary publication:
Solid-state structural characterization of cutinase-ECE-pincer-metal hybrids.
Rutten L, Wieczorek B, Mannie JP, Kruithof CA, Dijkstra HP, Egmond MR, Lutz M, Klein Gebbink RJ, Gros P, van Koten G
Chemistry 15 4270-80 (2009)
PMID: 19219875

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 22.29 KDa
Source organism: Fusarium vanettenii
Expression system: Escherichia coli
UniProt:
  • Canonical: P00590 (Residues: 17-230; Coverage: 100%)
Gene names: CUT1, CUTA
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand NXC 2 x NXC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 33.97Å b: 71.859Å c: 68.568Å
α: 90° β: 96.94° γ: 90°
R-values:
R R work R free
0.202 0.199 0.253
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Cutinase: characteristics, preparation, and application.
Chen et al. (2013)
1 more

1 mention without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)