› 3esh

X-ray diffraction
2.5Å resolution

Crystal structure of a probable metal-dependent hydrolase from Staphylococcus aureus. Northeast Structural Genomics target ZR314

Released:
DOI: 10.2210/pdb3esh/pdb
PDB entry 3esh coloured by chain, front view.
PDB entry 3esh coloured by chain, side view.
PDB entry 3esh coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Entry authors: Seetharaman J, Chen Y, Wang H, Janjua H, Foote EL, Xiao R, Nair R, Everett JK, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 280 amino acids
Theoretical weight: 32.64 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3JZW1 (Residues: 1-280; Coverage: 100%)
Gene name: SAV1747
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 27 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P42212
Unit cell:
a: 113.11Å b: 113.11Å c: 155.807Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.274
Expression system: Escherichia coli

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