› 3esl

X-ray diffraction
1.74Å resolution

Crystal structure of the conserved N-terminal domain of the mitotic checkpoint component BUB1

Released:
DOI: 10.2210/pdb3esl/pdb
PDB entry 3esl coloured by chain, front view.
PDB entry 3esl coloured by chain, side view.
PDB entry 3esl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores.
Bolanos-Garcia VM, Kiyomitsu T, D'Arcy S, Chirgadze DY, Grossmann JG, Matak-Vinkovic D, Venkitaraman AR, Yanagida M, Robinson CV, Blundell TL
Structure 17 105-16 (2009)
PMID: 19141287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Budding uninhibited by benzimidazole-related protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 24.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A6ZUJ9 (Residues: 29-230; Coverage: 20%)
Gene names: BUB1, SCY_2082
Sequence domains: Mad3/BUB1 homology region 1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NHE 2 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 130.304Å b: 59.784Å c: 71.299Å
α: 90° β: 97.96° γ: 90°
R-values:
R R work R free
0.19 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Congenital microcephaly.
Alcantara et al. (2014)
14 more

6 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
5 more