› 3esv

X-ray diffraction
2Å resolution

Crystal structure of the engineered neutralizing antibody M18

Released:
DOI: 10.2210/pdb3esv/pdb
PDB entry 3esv coloured by chain, front view.
PDB entry 3esv coloured by chain, side view.
PDB entry 3esv coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen.
Leysath CE, Monzingo AF, Maynard JA, Barnett J, Georgiou G, Iverson BL, Robertus JD
J Mol Biol 387 680-93 (2009)
PMID: 19361425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antibody M18 light chain and antibody M18 heavy chain linked with a synthetic (GGGGS)4 linker Chains: F, G
Molecule details ›
Chains: F, G
Length: 252 amino acids
Theoretical weight: 26.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P1
Unit cell:
a: 36.07Å b: 54.21Å c: 61.95Å
α: 71.8° β: 75.58° γ: 71.25°
R-values:
R R work R free
0.194 0.194 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Immunity to ricin: fundamental insights into toxin-antibody interactions.
O'Hara et al. (2012)
4 more

3 mentions without citation

Antibody repertoire deep sequencing reveals antigen-independent selection in maturing B cells.
Kaplinsky et al. (2014)
2 more