› 3etj

X-ray diffraction
1.6Å resolution

Crystal structure E. coli Purk in complex with Mg, ADP, and Pi

Released:
DOI: 10.2210/pdb3etj/pdb
PDB entry 3etj coloured by chain, front view.
PDB entry 3etj coloured by chain, side view.
PDB entry 3etj coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli.
Thoden JB, Holden HM, Firestine SM
Biochemistry 47 13346-53 (2008)
PMID: 19053251

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 355 amino acids
Theoretical weight: 39.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P09029 (Residues: 1-355; Coverage: 100%)
Gene names: JW0511, b0522, purK
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 4 x MG
Ligand PI 2 x PI
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 128.8Å b: 70.5Å c: 88.7Å
α: 90° β: 124.3° γ: 90°
R-values:
R R work R free
0.16 0.158 0.215
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Unlocking the sugar "steric gate" of DNA polymerases.
Brown et al. (2011)
5 more

4 mentions without citation

Structural analysis of the active site geometry of N5-carboxyaminoimidazole ribonucleotide synthetase from Escherichia coli.
Thoden et al. (2008)
3 more