› 3etn

X-ray diffraction
1.7Å resolution

Crystal structure of putative phosphosugar isomerase involved in capsule formation (YP_209877.1) from Bacteroides fragilis NCTC 9343 at 1.70 A resolution

Released:
DOI: 10.2210/pdb3etn/pdb
PDB entry 3etn coloured by chain, front view.
PDB entry 3etn coloured by chain, side view.
PDB entry 3etn coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Primary publication:
Structural analysis of arabinose-5-phosphate isomerase from Bacteroides fragilis and functional implications.
Chiu HJ, Grant JC, Farr CL, Jaroszewski L, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Wilson IA
Acta Crystallogr D Biol Crystallogr 70 2640-51 (2014)
PMID: 25286848

Function and Biology Details

Reaction catalysed: 
D-arabinose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar isomerase SIS-domain protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 24.48 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LIW1 (Residues: 1-201; Coverage: 100%)
Gene name: BF9343_0136
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

2 bound ligands:
Ligand CMK 4 x CMK
Ligand EDO 9 x EDO
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 60.409Å b: 114.267Å c: 115.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.197
Expression system: Escherichia coli

Quick links

Citations

6 mentions without citation

Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography.
Gourlay et al. (2010)
5 more