› 3eu0

X-ray diffraction
2.7Å resolution

Crystal structure of the S-nitrosylated Cys215 of PTP1B

Released:
DOI: 10.2210/pdb3eu0/pdb
PDB entry 3eu0 coloured by chain, front view.
PDB entry 3eu0 coloured by chain, side view.
PDB entry 3eu0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation.
Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC
J Biol Chem 283 35265-72 (2008)
PMID: 18840608

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 38.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-282; Coverage: 65%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SNC 1 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+
Spacegroup: P3121
Unit cell:
a: 88.72Å b: 88.72Å c: 104.72Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.202 0.246
Expression system: Escherichia coli

Quick links

Citations

47 review citations

Nitric oxide and redox mechanisms in the immune response.
Wink et al. (2011)
46 more

5 mentions without citation

Structural analysis of cysteine S-nitrosylation: a modified acid-based motif and the emerging role of trans-nitrosylation.
Marino et al. (2010)
4 more