› 3evp

X-ray diffraction
1.45Å resolution

crystal structure of circular-permutated EGFP

Released:
DOI: 10.2210/pdb3evp/pdb
PDB entry 3evp coloured by chain, front view.
PDB entry 3evp coloured by chain, side view.
PDB entry 3evp coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural basis for calcium sensing by GCaMP2.
Wang Q, Shui B, Kotlikoff MI, Sondermann H
Structure 16 1817-27 (2008)
PMID: 19081058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.35 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-144; Coverage: 60%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 51.06Å b: 62.22Å c: 69.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.132 0.174
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
18 more

16 mentions without citation

Tracking RNA with light: selection, structure, and design of fluorescence turn-on RNA aptamers.
Trachman et al. (2019)
15 more