› 3ewb

X-ray diffraction
2.1Å resolution

Crystal structure of N-terminal domain of putative 2-isopropylmalate synthase from Listeria monocytogenes

Released:
DOI: 10.2210/pdb3ewb/pdb
PDB entry 3ewb coloured by chain, front view.
PDB entry 3ewb coloured by chain, side view.
PDB entry 3ewb coloured by chain, top view.
Source organism: Listeria monocytogenes serotype 4b str. F2365
Entry authors: Ramagopal UA, Toro R, Gilmore M, Hu S, Maletic M, Rodgers L, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180769 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-isopropylmalate synthase Chain: X
Molecule details ›
Chain: X
Length: 293 amino acids
Theoretical weight: 32.69 KDa
Source organism: Listeria monocytogenes serotype 4b str. F2365
Expression system: Escherichia coli
UniProt:
  • Canonical: Q71Y35 (Residues: 2-283; Coverage: 55%)
Gene names: LMOf2365_2010, leuA
Sequence domains: HMGL-like
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 72.063Å b: 72.063Å c: 106.622Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.245
Expression system: Escherichia coli

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