› 3ex9

X-ray diffraction
2.2Å resolution

Crystal structure of PhzA/B from Burkholderia cepacia R18194 crystallized in C2221

Released:
DOI: 10.2210/pdb3ex9/pdb
PDB entry 3ex9 coloured by chain, front view.
PDB entry 3ex9 coloured by chain, side view.
PDB entry 3ex9 coloured by chain, top view.
Source organism: Burkholderia lata
Primary publication:
PhzA/B catalyzes the formation of the tricycle in phenazine biosynthesis.
Ahuja EG, Janning P, Mentel M, Graebsch A, Breinbauer R, Hiller W, Costisella B, Thomashow LS, Mavrodi DV, Blankenfeldt W
J Am Chem Soc 130 17053-61 (2008)
PMID: 19053436

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenazine biosynthesis protein A/B Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 21.53 KDa
Source organism: Burkholderia lata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q396C9 (Residues: 1-165; Coverage: 100%)
Gene name: Bcep18194_B1568
Sequence domains: Phenazine biosynthesis protein A/B
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 78.94Å b: 79.68Å c: 64.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.24
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Genomics of secondary metabolite production by Pseudomonas spp.
Gross et al. (2009)
10 more

1 mention without citation

Structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in Catenulispora acidiphila.
Filippova et al. (2014)