3exc

X-ray diffraction
2.25Å resolution

Structure of the RNA'se SSO8090 from Sulfolobus solfataricus

Released:
Source organism: Saccharolobus solfataricus
Entry authors: Singer AU, Skarina T, Tan K, Kagan O, Onopriyenko O, Edwards AM, Joachimiak A, Yakunin AF, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-189037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas2 2 Chain: X
Molecule details ›
Chain: X
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Y85 (Residues: 1-88; Coverage: 100%)
Gene names: SSO8090, cas22
Sequence domains: CRISPR associated protein Cas2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 93.479Å b: 93.479Å c: 48.596Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.221
Expression system: Escherichia coli