› 3ey2

X-ray diffraction
1.04Å resolution

A Conformational Transition in the Structure of a 2'-Thiomethyl-Modified DNA Visualized at High Resolution

Released:
DOI: 10.2210/pdb3ey2/pdb
PDB entry 3ey2 coloured by chain, front view.
PDB entry 3ey2 coloured by chain, side view.
PDB entry 3ey2 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution.
Pallan PS, Prakash TP, Li F, Eoff RL, Manoharan M, Egli M
Chem Commun (Camb) 2017-9 (2009)
PMID: 19333476

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116225 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*GP*CP*GP*TP*AP*(USM)P*AP*CP*GP*C)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 10 nucleotides
Theoretical weight: 3.08 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand N3D 1 x N3D
1 modified residue:
Ligand USM 2 x USM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 24.69Å b: 42.92Å c: 46.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.125 0.153
Expression system: Not provided

Quick links

Citations

2 review citations

Crystallographic studies of chemically modified nucleic acids: a backward glance.
Egli et al. (2010)
1 more

1 mention without citation

The many twists and turns of DNA: template, telomere, tool, and target.
Egli et al. (2010)