› 3ey3

X-ray diffraction
1.25Å resolution

A Conformational Transition in the Structure of a 2'-Thiomethyl-Modified DNA Visualized at High Resolution

Released:
DOI: 10.2210/pdb3ey3/pdb
PDB entry 3ey3 coloured by chain, front view.
PDB entry 3ey3 coloured by chain, side view.
PDB entry 3ey3 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution.
Pallan PS, Prakash TP, Li F, Eoff RL, Manoharan M, Egli M
Chem Commun (Camb) 2017-9 (2009)
PMID: 19333476

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116226 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*CP*GP*CP*GP*AP*AP*(USM)P*(USM)P*CP*GP*CP*G)-3' Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SR 7 x SR
Ligand MPD 2 x MPD
1 modified residue:
Ligand USM 8 x USM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 40.074Å b: 43.218Å c: 88.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.147 0.207
Expression system: Not provided

Quick links

Citations

2 review citations

Crystallographic studies of chemically modified nucleic acids: a backward glance.
Egli et al. (2010)
1 more

1 mention without citation

The many twists and turns of DNA: template, telomere, tool, and target.
Egli et al. (2010)