› 3eyi

X-ray diffraction
1.45Å resolution

The crystal structure of the second Z-DNA binding domain of human DAI (ZBP1) in complex with Z-DNA

Released:
DOI: 10.2210/pdb3eyi/pdb
PDB entry 3eyi coloured by chain, front view.
PDB entry 3eyi coloured by chain, side view.
PDB entry 3eyi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the second Z-DNA binding domain of human DAI (ZBP1) in complex with Z-DNA reveals an unusual binding mode to Z-DNA.
Ha SC, Kim D, Hwang HY, Rich A, Kim YG, Kim KK
Proc Natl Acad Sci U S A 105 20671-6 (2008)
PMID: 19095800

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116227 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Z-DNA-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H171 (Residues: 103-166; Coverage: 15%)
Gene names: C20orf183, DLM1, ZBP1
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-TCGCGCG-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 29.53Å b: 58.249Å c: 88.609Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.153 0.194
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

ZBP1: Innate Sensor Regulating Cell Death and Inflammation.
Kuriakose et al. (2018)
20 more

11 mentions without citation

Molecular and Structural Basis of DNA Sensors in Antiviral Innate Immunity.
Zahid et al. (2020)
10 more