› 3eyo

X-ray diffraction
2.5Å resolution

Crystal structure of anti-human cytomegalovirus antibody 8F9

Released:
DOI: 10.2210/pdb3eyo/pdb
PDB entry 3eyo coloured by chain, front view.
PDB entry 3eyo coloured by chain, side view.
PDB entry 3eyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus.
Thomson CA, Bryson S, McLean GR, Creagh AL, Pai EF, Schrader JW
EMBO J 27 2592-602 (2008)
PMID: 18772881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171204 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 216 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PJF2 (Residues: 21-114, 115-235; Coverage: 100%)
Gene name: IGK@
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 242 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ZCG9 (Residues: 3-105, 106-106, 107-121; Coverage: 98%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 63.03Å b: 110.73Å c: 180.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.268
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rich et al. (2010)
8 more

4 mentions without citation

Broadly neutralizing antibodies from an individual that naturally cleared multiple hepatitis C virus infections uncover molecular determinants for E2 targeting and vaccine design.
Keck et al. (2019)
3 more