› 3eyy

X-ray diffraction
2.4Å resolution

Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition

Released:
DOI: 10.2210/pdb3eyy/pdb
PDB entry 3eyy coloured by chain, front view.
PDB entry 3eyy coloured by chain, side view.
PDB entry 3eyy coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition.
An YJ, Ahn BE, Han AR, Kim HM, Chung KM, Shin JH, Cho YB, Roe JH, Cha SS
Nucleic Acids Res 37 3442-51 (2009)
PMID: 19336416

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron uptake regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.25 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K4F8 (Residues: 1-145; Coverage: 100%)
Gene names: SCD66.17, SCO4180
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NI 2 x NI
Ligand ZN 2 x ZN
Ligand CL 5 x CL
Ligand EDO 4 x EDO
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P31
Unit cell:
a: 79.164Å b: 79.164Å c: 49.732Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.264
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
10 more

8 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
7 more