Function and Biology Details
Reaction catalysed:
Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Phosphocarrier protein HPr-like
- HPr-like superfamily
- PtsI, HPr-binding domain superfamily
- Phosphotransferase system, HPr serine phosphorylation site
- Phosphotransferase system, HPr histidine phosphorylation site
- PEP-utilising enzyme, active site
- PEP-utilising enzyme, mobile domain
- Phosphohistidine domain superfamily
1 more domain
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140393 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphoenolpyruvate-protein phosphotransferase
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 27.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 249 amino acids
Theoretical weight: 27.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P08839 (Residues: 1-249; Coverage: 43%)
Sequence domains:
Structure domains:
Phosphocarrier protein HPr
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
simulated annealing
Expression system: Escherichia coli
