› 3f0w

X-ray diffraction
2.7Å resolution

Human NUMB-like protein, phosphotyrosine interaction domain

Released:
DOI: 10.2210/pdb3f0w/pdb
PDB entry 3f0w coloured by chain, front view.
PDB entry 3f0w coloured by chain, side view.
PDB entry 3f0w coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Lehtio L, Moche M, Andersson J, Arrowsmith CH, Berglund H, Bountra C, D Busam R, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Karlberg T, Kotenyova T, Nilsson ME, Nyman T, Persson C, Sagemark J, Schueler H, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Welin M, Wikstrom M, Wisniewska M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Numb-like protein Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6R0 (Residues: 60-204; Coverage: 24%)
Gene name: NUMBL
Sequence domains: Phosphotyrosine interaction domain (PTB/PID)
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 55.15Å b: 55.15Å c: 99.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.238 0.281
Expression system: Escherichia coli

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