› 3f0x

X-ray diffraction
2.6Å resolution

Staphylococcus aureus F98Y mutant dihydrofolate reductase complexed with NADPH and 2,4-Diamino-5-[3-(3-methoxy-5-(3,5-dimethylphenyl)phenyl)but-1-ynyl]-6-methylpyrimidine

Released:
DOI: 10.2210/pdb3f0x/pdb
PDB entry 3f0x coloured by chain, front view.
PDB entry 3f0x coloured by chain, side view.
PDB entry 3f0x coloured by chain, top view.
Source organism: Staphylococcus aureus RF122
Entry authors: Anderson AC, Frey KM, Liu J, Lombardo MN

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimethoprim-sensitive dihydrofolate reductase Chain: X
Molecule details ›
Chain: X
Length: 157 amino acids
Theoretical weight: 18.03 KDa
Source organism: Staphylococcus aureus RF122
Expression system: Escherichia coli
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand NDP 1 x NDP
Ligand 53T 1 x 53T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 79.048Å b: 79.048Å c: 108.727Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.255
Expression system: Escherichia coli

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