› 3f1j

X-ray diffraction
2.65Å resolution

Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals RNA binding properties

Released:
DOI: 10.2210/pdb3f1j/pdb
PDB entry 3f1j coloured by chain, front view.
PDB entry 3f1j coloured by chain, side view.
PDB entry 3f1j coloured by chain, top view.
Source organism: Borna disease virus
Primary publication:
Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties.
Neumann P, Lieber D, Meyer S, Dautel P, Kerth A, Kraus I, Garten W, Stubbs MT
Proc Natl Acad Sci U S A 106 3710-5 (2009)
PMID: 19237566

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.37 KDa
Source organism: Borna disease virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0C794 (Residues: 1-142; Coverage: 100%)
Gene name: M
Sequence domains: ssRNA-binding matrix protein of Bornaviridae
Structure domains: Borna disease virus, matrix protein

Ligands and Environments

2 bound ligands:
Ligand C5P 1 x C5P
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: I432
Unit cell:
a: 143.57Å b: 143.57Å c: 143.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.264
Expression system: Escherichia coli BL21

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Citations

8 review citations

Paramyxovirus glycoprotein incorporation, assembly and budding: a three way dance for infectious particle production.
El Najjar et al. (2014)
7 more

4 mentions without citation

Conformational plasticity of the Ebola virus matrix protein.
Radzimanowski et al. (2014)
3 more