› 3f1w

X-ray diffraction
2.9Å resolution

Crystal structure of a mutant proliferating cell nuclear antigen that blocks translesion synthesis

Released:
DOI: 10.2210/pdb3f1w/pdb
PDB entry 3f1w coloured by chain, front view.
PDB entry 3f1w coloured by chain, side view.
PDB entry 3f1w coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a mutant form of proliferating cell nuclear antigen that blocks translesion DNA synthesis.
Freudenthal BD, Ramaswamy S, Hingorani MM, Washington MT
Biochemistry 47 13354-61 (2008)
PMID: 19053247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-147505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.97 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P213
Unit cell:
a: 123.127Å b: 123.127Å c: 123.127Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Many Roles of PCNA in Eukaryotic DNA Replication.
Boehm et al. (2016)
1 more

5 mentions without citation

PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA.
Dieckman et al. (2012)
4 more