› 3f2h

X-ray diffraction
2Å resolution

Crystal structure of the mercury-bound form of MerB mutant C160S, the Organomercurial Lyase involved in a bacterial mercury resistance system

Released:
DOI: 10.2210/pdb3f2h/pdb
PDB entry 3f2h coloured by chain, front view.
PDB entry 3f2h coloured by chain, side view.
PDB entry 3f2h coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the organomercurial lyase MerB in its free and mercury-bound forms: insights into the mechanism of methylmercury degradation.
Lafrance-Vanasse J, Lefebvre M, Di Lello P, Sygusch J, Omichinski JG
J Biol Chem 284 938-44 (2009)
PMID: 19004822

Function and Biology Details

Reaction catalysed: 
An alkylmercury + H(+) = an alkane + Hg(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand HG 2 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 38.88Å b: 89.169Å c: 54.627Å
α: 90° β: 97.45° γ: 90°
R-values:
R R work R free
0.196 0.191 0.24
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacterial mer operon-mediated detoxification of mercurial compounds: a short review.
Mathema et al. (2011)
1 more