› 3f2i

X-ray diffraction
2Å resolution

Crystal structure of the alr0221 protein from Nostoc, Northeast Structural Genomics Consortium Target NsR422.

Released:
DOI: 10.2210/pdb3f2i/pdb
PDB entry 3f2i coloured by chain, front view.
PDB entry 3f2i coloured by chain, side view.
PDB entry 3f2i coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Forouhar F, Lew S, Seetharaman J, Sahdev S, Xiao R, Foote EL, Ciccosanti C, Belote RL, Nair R, Everett JK, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187150 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphohistidine phosphatase SixA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 172 amino acids
Theoretical weight: 19.86 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z077 (Residues: 1-164; Coverage: 100%)
Gene name: alr0221
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand PO4 6 x PO4
Ligand CL 6 x CL
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 65.099Å b: 94.329Å c: 92.062Å
α: 90° β: 93.64° γ: 90°
R-values:
R R work R free
0.197 0.196 0.24
Expression system: Escherichia coli

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