› 3f2z

X-ray diffraction
1.3Å resolution

Crystal structure of the C-terminal domain of a chitobiase (BF3579) from Bacteroides fragilis, Northeast Structural Genomics Consortium Target BfR260B

Released:
DOI: 10.2210/pdb3f2z/pdb
PDB entry 3f2z coloured by chain, front view.
PDB entry 3f2z coloured by chain, side view.
PDB entry 3f2z coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Forouhar F, Lew S, Seetharaman J, Janjua H, Xiao R, Foote EL, Ciccosanti C, Lee D, Nair R, Everett JK, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
uncharacterized protein BF3579 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.9 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L9G5 (Residues: 291-440; Coverage: 34%)
Gene name: BF9343_3481
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 47.893Å b: 51.684Å c: 63.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.175
Expression system: Escherichia coli

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